Figure 2.The LIM domains of TRIP6 interact with POT1∆C by yeast two-hybrid.(A) Top: His phenotypes of the B40 strain
carrying the pLexA-POT1∆C bait plasmid and the plasmids shown on the
right side, including the b12 positive clone and another recovered clone,
c3, which proved negative upon retransformation. Bottom: Patch LacZ assay
of the same strains, showing that the b12 clone activates the LacZ reporter
gene as well. (B) Liquid β-Gal assays using the B40 yeast
strain and the bait plasmids shown at the bottom, with the GAD vector or
GAD-b2 clone. The b2 clone activates LacZ with LexA-POT1 or
LexA-POT1∆C, but not with LexA-TRF1. The standard deviations were
calculated on three independent yeast colonies, each assayed three times.
