Figure 7.In reproductively mature WT yeast that entered the non-proliferative stationary (ST) phase under CR, LCA modulates mitochondrial morphology and functions, enhances stress resistance, attenuates mitochondria-controlled apoptosis, and increases stability of nuclear and mitochondrial DNA.(A)
Percent of WT cells grown in medium with or without LCA and exhibiting a
tubular mitochondrial network or fragmented mitochondria. Mitochondria were
visualized by indirect immunofluorescence microscopy using monoclonal
anti-porin primary antibodies and Alexa Fluor 568-conjugated goat
anti-mouse IgG secondary antibodies. (B - D) Oxygen
consumption by WT cells grown in medium with or without LCA (B),
their mitochondrial membrane potential ΔΨ (C) and their
ROS levels (D). ΔΨ and ROS were visualized in living cells by
fluorescence microscopy using fluorescent dyes Rhodamine 123 or
Dihydrorhodamine 123, respectively. (E) The resistance of WT cells
pre-grown in medium with or without LCA to chronic oxidative, thermal and
osmotic stresses. (F) Viability of WT cells pre-grown in medium with
or without LCA and then treated for 1 h with hydrogen peroxide or acetic
acid (AcOH) to induce mitochondria-controlled apoptosis. (G- I)
The frequencies of rho- and rho0mutations
in mitochondrial DNA (G), rib2 and rib3 mutations in
mitochondrial DNA (H), and of can1 (Canr)
mutations in nuclear DNA (I) of WT cells grown in medium with or without
LCA. Data in A - D and F - I are presented as
means ± SEM (n = 4-17; ***p < 0.001; **p <
0.01). WT cells grown on 0.2% glucose in the presence or absence of
LCA were taken for analyses at day 7, when they reached reproductive
maturation by entering into ST phase.
