Research Paper Volume 2, Issue 7 pp 393—414

Figure 6. In chronologically aging WT yeast that entered the non-proliferative stationary (ST) phase under CR, LCA alters the levels of lipids and protects cells from lipid-induced necrotic death. (A - C) Levels of triacylglycerols (TAG) and free fatty acids (FFA) measured by quantitative mass spectrometry (A and B, respectively) and of diacylglycerols (DAG) monitored by TLC (C) in WT cells grown in medium with or without LCA. (D - F) Viability of WT cells pre-grown in medium with or without LCA and then treated for 2 h with palmitoleic acid (D), oleic acid (E) or DiC8 diacylglycerol (F). (G - I) Percent of WT cells (pre-grown in medium with or without LCA) that following their treatment with palmitoleic acid (G), oleic acid (H) or DiC8 diacylglycerol (I) displayed Annexin V negative and PI positive (Annexin V^- and PI⁺) staining characteristic of necrotic cell death. Data are presented as means ± SEM (n = 3-9; ***p < 0.001; **p < 0.01). WT cells grown on 0.2% glucose in the presence or absence of LCA were taken for analyses at day 7, when they reached reproductive maturation by entering into ST phase.