Figure 6.In chronologically aging WT yeast that entered the non-proliferative stationary (ST) phase under CR, LCA alters the levels of lipids and protects cells from lipid-induced necrotic death.(A - C)
Levels of triacylglycerols (TAG) and free fatty acids (FFA) measured by
quantitative mass spectrometry (A and B, respectively) and of
diacylglycerols (DAG) monitored by TLC (C) in WT cells grown in
medium with or without LCA. (D - F) Viability of WT cells
pre-grown in medium with or without LCA and then treated for 2 h with
palmitoleic acid (D), oleic acid (E) or DiC8 diacylglycerol (F).
(G - I) Percent of WT cells (pre-grown in medium with or
without LCA) that following their treatment with palmitoleic acid (G),
oleic acid (H) or DiC8 diacylglycerol (I) displayed Annexin V
negative and PI positive (Annexin V- and PI+)
staining characteristic of necrotic cell death. Data are presented as means
± SEM (n = 3-9; ***p < 0.001; **p < 0.01).
WT cells grown on 0.2% glucose in the presence or absence of LCA were taken
for analyses at day 7, when they reached reproductive maturation by
entering into ST phase.
