Figure 3.A high-throughput screen of compound libraries for small molecules that extend the CLS of yeast under CR conditions.(A) A microplate assay for
measuring yeast CLS by monitoring optical density at 600 nm (OD600)
was used for screening representative compounds from several commercial
libraries for small molecules that extend the CLS of pex5Δ cells grown
under CR on 0.5% glucose. (B) The OD600 of a cell culture
in the replica microplate following incubation for 16 to 24 hours
correlates with the number of viable cells present in this culture before
it was taken from the master microplate for replica plating. (C) The
effect of various concentrations of the identified anti-aging small
molecules on the CLS of the pex5Δ (Δ) strain under CR
conditions. The "OD600 at day 14/OD600 at day 1"
ratio was used as a measure of CLS. Data are
presented as means ± SEM (n = 3-5; ***p < 0.001; **p < 0.01; *p <
0.05). The anti-aging small molecules LCA, AS, BT, NF and ID belong
to five chemical groups.
