Research Paper Volume 2, Issue 7 pp 393—414

Figure 3. A high-throughput screen of compound libraries for small molecules that extend the CLS of yeast under CR conditions. (A) A microplate assay for measuring yeast CLS by monitoring optical density at 600 nm (OD₆₀₀) was used for screening representative compounds from several commercial libraries for small molecules that extend the CLS of pex5Δ cells grown under CR on 0.5% glucose. (B) The OD₆₀₀ of a cell culture in the replica microplate following incubation for 16 to 24 hours correlates with the number of viable cells present in this culture before it was taken from the master microplate for replica plating. (C) The effect of various concentrations of the identified anti-aging small molecules on the CLS of the pex5Δ (Δ) strain under CR conditions. The "OD₆₀₀ at day 14/OD₆₀₀ at day 1" ratio was used as a measure of CLS. Data are presented as means ± SEM (n = 3-5; ***p < 0.001; **p < 0.01; *p < 0.05). The anti-aging small molecules LCA, AS, BT, NF and ID belong to five chemical groups.