Figure 5.SIRT1 prevents expression of endothelial adhesion molecules.
(A)
RelA/p65 DNA-binding in aortic nuclear extracts from ApoE-/-
SIRT1+/- (+/-, n=8, black column) is elevated in ApoE-/-
SIRT1+/+ (+/+, n=6, white column) mice. (B)
Expression levels of IL-1β, P-Selectin, and TNFα
in aortic lysates of ApoE-/- SIRT1+/+
(white columns) and ApoE-/- SIRT1+/- (black
columns) mice. n=8 per genotype. Enhanced expression of ICAM-1 (C)
and VCAM-1 (D)
is observed in non-atherosclerotic ApoE-/-
SIRT1+/- (+/- and black columns) compared with ApoE-/-
SIRT1+/+ (+/+ and white columns) aortae 3 h post
intra-peritoneal LPS (striped columns) injection. n=6 per genotype. Scale:
50 μm. *p<0.05; **p< 0.01.
