SIRT1 reduces endothelial activation without affecting vascular
                        function in ApoE-/- mice

Sokrates Stein; Nicola Schäfer; Alexander Breitenstein; Christian Besler; Stephan Winnik; Christine Lohmann; Kathrin Heinrich; Chad E. Brokopp; Christoph Handschin; Ulf Landmesser; Felix C. Tanner; Thomas F. Lüscher; Christian M. Matter

doi:doi:10.18632/aging.100162

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Research Paper Volume 2, Issue 6 pp 353—360

SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice

Figure 5. SIRT1 prevents expression of endothelial adhesion molecules. (A) RelA/p65 DNA-binding in aortic nuclear extracts from ApoE^-/- SIRT1^+/-(+/-, n=8, black column) is elevated in ApoE^-/- SIRT1^+/+ (+/+, n=6, white column) mice. (B) Expression levels of IL-1β, P-Selectin, and TNFα in aortic lysates of ApoE^-/- SIRT1^+/+ (white columns) and ApoE^-/- SIRT1^+/- (black columns) mice. n=8 per genotype. Enhanced expression of ICAM-1 (C) and VCAM-1 (D) is observed in non-atherosclerotic ApoE^-/- SIRT1^+/- (+/- and black columns) compared with ApoE^-/- SIRT1^+/+ (+/+ and white columns) aortae 3 h post intra-peritoneal LPS (striped columns) injection. n=6 per genotype. Scale: 50 μm. *p<0.05; **p< 0.01.