Research Paper Volume 2, Issue 6 pp 353—360
SIRT1 reduces endothelial activation without affecting vascular function in ApoE-/- mice
Figure 4. SIRT1 suppresses NF-κB signaling in HAECs. (A) RelA/p65 DNA binding is higher in HAEC pretreated with splitomicin (Splito) compared with the untreated group (-), 30 min after TNFα-stimulation. n=5. (B) RelA/p65 immunoprecipitation in HAECs reveals more acetyl-K310-RelA/p65 upon SIRT1-siRNA treatment 20 min after TNFα-stimulation compared to scrambled siRNA-treated cells. n=2. (C) Western blot showing SIRT1 silencing using siRNA (top), and VCAM-1 mRNA expression 5 h after TNFα stimulation in SIRT1-siRNA treated HAECs (graph). n=4. *p<0.05.