Figure 4.SIRT1 suppresses NF-κB signaling in HAECs. (A)
RelA/p65 DNA binding is higher in HAEC pretreated with splitomicin (Splito)
compared with the untreated group (-), 30 min after TNFα-stimulation.
n=5. (B)
RelA/p65 immunoprecipitation in HAECs reveals more acetyl-K310-RelA/p65
upon SIRT1-siRNA treatment 20 min after TNFα-stimulation compared to
scrambled siRNA-treated cells. n=2. (C) Western blot
showing SIRT1 silencing using siRNA (top), and VCAM-1 mRNA expression 5 h
after TNFα stimulation in SIRT1-siRNA treated HAECs (graph). n=4.
*p<0.05.
