Research Paper Volume 2, Issue 6 pp 344—352
The choice between p53-induced senescence and quiescence is determined in part by the mTOR pathway
Figure 5. Effect of rapamycin on nutlin-induced senescence in melanoma cells . (A. ) ) Immunoblot. MEF cells were incubated with 10 nutlin-3a with or without 10 nM rapamycin for 1 day and immunoblot using rabbit anti-phospho-S6 (Ser240/244) and (Ser235/236) and mouse anti-S6 was performed. (B) Beta-Gal staining. MEF cells were incubated with 10 uM nutlin alone or with 500 nM rapamycin for 4 days, then drugs were washed out and cells were incubated for additional 4 days and stained for beta-Gal. Bars 50 um.