Figure 5.Effect of rapamycin on nutlin-induced senescence in melanoma cells . (A.) ) Immunoblot.
MEF cells were incubated with 10 nutlin-3a with or without 10 nM rapamycin
for 1 day and immunoblot using rabbit anti-phospho-S6 (Ser240/244) and
(Ser235/236) and mouse anti-S6 was performed. (B) Beta-Gal staining. MEF cells were incubated
with 10 uM nutlin alone or with 500 nM rapamycin for 4 days, then drugs
were washed out and cells were incubated for additional 4 days and stained
for beta-Gal. Bars 50 um.
