Figure 3.Effects of nutlin-3a and rapamycin on melanoma cells. (A) Mel-10 and Mel-9
cells were incubated with 10 uM nutlin (N) and 500 nM rapamycin (R) for 1
day and immunoblot was performed. (B) Mel-10 and Mel-9 cells were
incubated with 10 uM nutlin and 500 nM rapamycin for 4 days, then drugs
were washed out and cells were incubated for additional 4 days and stained
for beta-Gal. Bars 50 um.
