Research Paper Volume 2, Issue 5 pp 274—284
Cooperation of DNA-PKcs and WRN helicase in the maintenance of telomeric D-loops
Figure 4. Effect of DNA-PKcs on WRN helicase activity on telomeric and non-telomeric D-loops. WRN wild type (WT) (3.3 nM, lanes 5, 6, 12, and 13) or WRN (E84A) (3.3 nM, lanes 3, 4, 10, and 11) was preincubated with DNA-PKcs (16.7 nM, lanes 4, 6, 11, and 13). A telomeric (lanes 2-6) or a non-telomeric D-loop substrate (lanes 9-13) was added to the reaction. Lanes 1 and 8: A DNA ladder marker. Lanes 7 and 14: heat-denatured telomeric and non-telomeric D-loop substrates, respectively, denoted by filled triangles.