Research Paper Volume 2, Issue 2 pp 93—100

Figure 1. DNA from aged subjects is more immunogenic than DNA from young subjects. (A) DCs were activated with aged and young DNA complexed with lipofectamine. The expression of costimulatory molecules CD80 and CD86 and the maturation molecule (CD83) in the unactivated and activated DCs was measured by flow cytometry. Figure is representative of ten such experiments using fifteen separate aged and young DNA. (B) Bar graph represents the mean fluorescence intensity of CD80, CD86 and CD83 of the same. (C) Supernatants collected from DCs activated with aged and young DNA were assayed for IFN-α using specific ELISA. Bar diagrams depict the concentration of IFN-α secreted by the DCs. Figure is mean + S.E. of thirty separate aged and young DNA tested.