Figure 1.DNA from aged subjects is more immunogenic than DNA from young subjects.(A) DCs
were activated with aged and young DNA complexed with lipofectamine. The
expression of costimulatory molecules CD80 and CD86 and the maturation
molecule (CD83) in the unactivated and activated DCs was measured by flow
cytometry. Figure is representative of ten such experiments using fifteen
separate aged and young DNA. (B) Bar graph represents the
mean fluorescence intensity of CD80, CD86 and CD83 of the same. (C)
Supernatants collected from DCs activated with aged and young DNA were
assayed for IFN-α using specific
ELISA. Bar diagrams depict the concentration of IFN-α secreted by the DCs. Figure is
mean +
S.E. of thirty separate aged and young DNA tested.
