Review Volume 2, Issue 1 pp 7—27

Figure 5. Clock gene expression in the liver under various feeding and lighting conditions in αMUPA (M) and WT mice. Expression levels of the following clock genes are presented under light-dark or disruptive light conditions:mPer2during ad libitum (AL) feeding, mCry1 under RF, and Bmal1 under IF.Total RNA extracted from liver tissue collected every 3 h around the circadian cycle (mean ± SEM; n=3 for each time-point and each mouse group) was reverse transcribed and analyzed by quantitative real time PCR. Clock gene levels were normalized using Gapdh as the reference gene.