Figure S1.Induction of p21 by IPTG. HT-p21 cells were plated in
6 well plates and treated with IPTG with or without rapamycin as indicated.
The next day, cells were lysed and immunoblot for p-S6, S6 and p21 was
performed as described in Methods. IPTG dramatically induced p21, without
affecting S6 phosphorylation, whereas rapamycin inhibited S6
phosphorylation, without affecting p21 induction.
