Research Paper Volume 1, Issue 12 pp 1008—1016

Figure 8. Loss of hypertrophy during proliferation of competent cells. 500 HT-p21 cells were plated in 12 well plates. The next day, either IPTG alone or IPTG plus rapamycin were added. After 3 days, plates were washed (I/w and I+R/w) or left unwashed. GFP per well was measured and cells were counted at days 1, 3, 6 and 9. GFP per cell was calculated (upper panel). Results are shown in arbitrary units (M±m). Open and closed squares: IPTG and IPTG plus Rapa, respectively. Open and closed circles: IPTG washed (I/w) and IPTG plus Rapa washed (I+R/w), respectively. When cells resumed exponential proliferation, GFP per cell dropped to normal levels. Due to robust proliferation, there was an increase of GFP per well.