Figure 4 — Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential

Research Paper|Volume 1, Issue 12|pp. 1008–1016

Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential

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Figure 4.Visualization of cellular hypertrophy. HT-p21 cells express enhanced green fluorescent protein (GFP) under the constitutive viral CMV promoter. Expression of GFP per cell is a marker of cellular hypertrophy. Low cell density - 2 thousand cells were plated in 100 mm dish and treated with either IPTG or IPTG + Rapamycin.