Figure 8.Effect of BMP-4 on VEGF-A and PEDF secretion from polarized RPE.Fold change over
control values calculated from ELISA analysis is presented to account for
inter donor variations. (A) The increase in VEGF-A secretion after
treatment with BMP-4 from the apical domain was not statistically
significant (p>0.05). (B) An increase in VEGF-A secretion from
the basolateral domain was found even with the lowest dose used (10ng/ml)
which increased further in a dose-dependent fashion. Asterisk indicates
that VEGF-A secretion with 75 and 100ng/ml BMP-4 treatment was
significantly higher than that of control (p<0.05). (C) The
cellular levels of VEGF-A were not significantly affected by BMP-4
treatment. (D, E, F) No significant change was observed for PEDF
secretion either at the apical domain or the basolateral domain and in
cellular PEDF levels. Data are mean±SEM from four different donors.
