Research Paper Volume 2, Issue 1 pp 28—42

Figure 8. Effect of BMP-4 on VEGF-A and PEDF secretion from polarized RPE. Fold change over control values calculated from ELISA analysis is presented to account for inter donor variations. (A) The increase in VEGF-A secretion after treatment with BMP-4 from the apical domain was not statistically significant (p>0.05). (B) An increase in VEGF-A secretion from the basolateral domain was found even with the lowest dose used (10ng/ml) which increased further in a dose-dependent fashion. Asterisk indicates that VEGF-A secretion with 75 and 100ng/ml BMP-4 treatment was significantly higher than that of control (p<0.05). (C) The cellular levels of VEGF-A were not significantly affected by BMP-4 treatment. (D, E, F) No significant change was observed for PEDF secretion either at the apical domain or the basolateral domain and in cellular PEDF levels. Data are mean±SEM from four different donors.