Figure 2.Differences in PEDF and VEGF secretion between nonpolarized and polarized RPE from various donors after 24h. Secretion from the polarized RPE cells
represent the sum of experimentally determined apical and basolateral
secretion values, normalized for total cellular protein. The total
secretion increased 34 fold for PEDF and 5.7 fold for VEGF-A (A).
Analysis of cellular protein (B) and mRNA (C) showed that
expression in polarized human RPE was higher compared to nonpolarized RPE
cells.
