Research Paper Volume 2, Issue 1 pp 28—42
Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Figure 1. Confocal and electron microscopic characterization of polarized RPE cells. Evidence for tight junction proteins and polarity in fetal RPE cells cultured on Transwell filters for 6 weeks. (A, B) Immunofluorescence staining of tight junction proteins ZO-1 and occludin. (C) Localization of Na/K- ATPase to the apical plasma membrane as shown in the confocal vertical (X-Z) section (white arrow). (D) Well differentiated apical microvilli observed by scanning electron microscopy (SEM). (E) Well developed microvilli (mv), localization of pigment on the apical side (asterisks), nuclei on basal side (N), and presence of tight-junctional complexes (arrows) by transmission electron microscopy (TEM).