Figure 10.SirT1 is necessary for promoter-dependent mIGF-1-dependent upregulation of anti-oxidant and hypertrophic genes adiponectin, UCP1 and MT-2. HL-1
cardiomyocytes were transfected with the indicated plasmids, and/or treated
with 20 ng/ml IGF-1 for 24 h. HL-1 cardiomyocytes were also co-transfected
with 1 μg of plasmids carrying Firefly luciferase under the control of
promoters of adiponectin (Adipo-Luc), UCP1 (UCP1-Luc) and MT-2 (MT-2-Luc)
genes, respectively, together with 1 μg of Renilla Luciferase plasmid.
Untransfected cells were used as control. Dual luciferase
assays were performed in duplicate for each condition. Results are means ± SE of 3 independent experiments
(*,**,***p versus untransfected/unstimulated
control cells).
