Research Paper Volume 2, Issue 1 pp 43—62

Figure 10. SirT1 is necessary for promoter-dependent mIGF-1-dependent upregulation of anti-oxidant and hypertrophic genes adiponectin, UCP1 and MT-2. HL-1 cardiomyocytes were transfected with the indicated plasmids, and/or treated with 20 ng/ml IGF-1 for 24 h. HL-1 cardiomyocytes were also co-transfected with 1 μg of plasmids carrying Firefly luciferase under the control of promoters of adiponectin (Adipo-Luc), UCP1 (UCP1-Luc) and MT-2 (MT-2-Luc) genes, respectively, together with 1 μg of Renilla Luciferase plasmid. Untransfected cells were used as control. Dual luciferase assays were performed in duplicate for each condition. Results are means ± SE of 3 independent experiments (^*,**,***p versus untransfected/unstimulated control cells).