Figure 2. Transcriptional
activity of p53 target genes in senescent WI-38 fibroblasts. (A)
Basal transcription of p53 target genes is not compromised in senescent
cells. Total RNA from early passage and senescent WI-38 cells was isolated
and the expression of specific mRNAs was determined by quantitative PCR.
Expression levels of each individual mRNA (from early passage and senescent
cells) were normalized to 18S rRNA. Expression levels in senescent cells
were calculated as fold change from the expression levels in early passage
cells. The standard deviation (SD) was calculated from four independent
experiments. (B) Basal expression levels of p53-regulated microRNA in
senescent cells. Expression levels of individual microRNAs from early
passage and senescent cells were determined by quantitative PCR, and
normalized to RNU48 as an internal control. Expression levels in senescent
cells were calculated and presented as in (A).
