Research Paper Volume 1, Issue 10 pp 845—854
Reduced transcriptional activity in the p53 pathway of senescent cells revealed by the MDM2 antagonist nutlin-3
Figure 1. WI-38 cells cease to proliferate after extensive in vitro passaging and become senescent. (A) Cell cycle analysis after BrdU incorporation in early passage and senescent cells. S phase cells are within the rectangle. (B) Senescent WI-38 cells stain for SA-β-Gal activity. Scale bar is 50 μm. (C) SAHF in senescent WI-38 cells. Early passage and senescent cells were immunostained with anti-HP-1γ antibody (green) and counter stained with DAPI (red). Scale bar is 20 μm.