Figure 1. WI-38 cells
cease to proliferate after extensive in vitro passaging and become
senescent. (A) Cell cycle
analysis after BrdU incorporation in early passage and senescent cells. S
phase cells are within the rectangle. (B) Senescent WI-38 cells
stain for SA-β-Gal activity.
Scale bar is 50 μm. (C)
SAHF in senescent WI-38 cells. Early passage and senescent cells were
immunostained with anti-HP-1γ antibody (green) and counter stained
with DAPI (red). Scale bar is 20 μm.
