Figure 4.TTP-mediated inhibition of hTERT expression.(A) hTERT
expression in HeLa Tet-Off/TTP-Flag cells growing in absence or presence of
TTP for 48 hr was examined by RT-PCR analysis (top panel) and western blot
using nuclear lysates (bottom panel). GAPDH and nucleoporin were detected
as loading controls, respectively. (B) TRAP assay showing inhibition
of telomerase activity in TTP-expressing cells. 0.5 and 1 μg of lysate from
cells grown in the absence or presence of TTP was used for TRAP assay as
described in Methods. Control reaction lacks Taq polymerase.
