Research Paper Volume 1, Issue 9 pp 803—817
The mRNA decay factor tristetraprolin (TTP) induces senescence in human papillomavirus-transformed cervical cancer cells by targeting E6-AP ubiquitin ligase
Figure 3. Enhanced p53 activity in HeLa cells expressing TTP. (A) Immunofluorescent detection of p53, shown in green, in HeLa Tet-Off/TTP-Flag cellsin the absence or presence of TTP for 48 hr. DAPI nuclear staining and merged images are shown. (B) Expression of TTP induces p53 transcriptional activity. Luciferase reporter constructs containing either a p53-dependent promoter (pp53-Luciferase) or control vector (pTA-Luciferase) were transfected into HeLa Tet-Off/TTP-Flag cells and allowed to grow without (grey bars) or with (black bars) TTP induction for 48 hr. Relative activity was assessed as luciferase activity normalized to renilla activity and are the averages of 3 experiments. (*) P < 0.01