Figure 3.Enhanced p53 activity in HeLa cells expressing TTP.(A)
Immunofluorescent detection of p53, shown in green, in HeLa
Tet-Off/TTP-Flag cellsin the absence or
presence of TTP for 48 hr. DAPI nuclear staining and merged images are
shown. (B) Expression of TTP induces p53 transcriptional activity.
Luciferase reporter constructs containing either a p53-dependent promoter
(pp53-Luciferase) or control vector (pTA-Luciferase) were transfected into
HeLa Tet-Off/TTP-Flag cells and allowed to grow without (grey bars) or with
(black bars) TTP induction for 48 hr. Relative activity was assessed as
luciferase activity normalized to renilla activity and are the averages of
3 experiments. (*) P < 0.01
