Figure 9.Effects of 3-NPA, an inhibitor of CII (succinic dehydrogenase) activity on the phosphorylation of the p38 MAPK catalytic site in fibroblasts from young (4-6 mos) and old (21-24 mos) wild-type and dwarf mice. (A) The bar graphs and
western blot analyses below each bar graph show the phosphorylation
levels of the p38 MAPK catalytic site amino acid residues
(P-Thr180/P-Tyr182) in (A) young fibroblasts (4-6 mos) and
(B) old wild-type and dwarf fibroblasts after treatment with
5 mM or 20 mM 3-NPA. The fibroblasts from young dwarf mice
show an attenuated response to the 3-NPA-mediated phosphorylation
of the catalytic site (n=3). Both young and old wild-type and
dwarf fibroblast responses peak at 30 minutes. However, the
recovery of the old wild-type cells is delayed. The fibroblasts
from old dwarf mice also show resistance and rapid recovery
to 3-NPA treatment similar to the response shown by the young
dwarf fibroblasts. *represents p<0.05 between wild-type vs.
dwarf.
