Figure 8.The Ames dwarf fibroblasts show a decreased level of induction of p38 MAPK kinase activity in response to ROT.
Extracts from young wild type and dwarf fibroblasts were
used to measure the p38 MAPK kinase activity using the Thr71
residue of ATF-2 as its substrate. The bar graphs and
immunoblots show the effects of 5 μM and 20 μM ROT on the in
vitro p38 MAPK kinase activity as indicated by the level of
induction of phosphorylation of the Thr71 ATF-2 catalytic site
amino acid residue.
