Research Paper Volume 1, Issue 9 pp 784—802
Dermal fibroblasts from long-lived Ames dwarf mice maintain their in vivo resistance to mitochondrial generated reactive oxygen species (ROS)
Figure 8. The Ames dwarf fibroblasts show a decreased level of induction of p38 MAPK kinase activity in response to ROT. Extracts from young wild type and dwarf fibroblasts were used to measure the p38 MAPK kinase activity using the Thr71 residue of ATF-2 as its substrate. The bar graphs and immunoblots show the effects of 5 μM and 20 μM ROT on the in vitro p38 MAPK kinase activity as indicated by the level of induction of phosphorylation of the Thr71 ATF-2 catalytic site amino acid residue.