Research Paper Volume 1, Issue 9 pp 784—802
Dermal fibroblasts from long-lived Ames dwarf mice maintain their in vivo resistance to mitochondrial generated reactive oxygen species (ROS)
Figure 4. The pool levels of total thioredoxin and re-duced thioredoxin in fibroblasts derived from young wild type and dwarf mice. Cytoplasmic extracts (100 μg) of young wild type and dwarf fibroblasts were either untreated or treated with 4-acetamido-4'-maleimideyl-stilbene-2,2'-disulfonic acid (AMS) for 2 hours, resolved by15% SDS-PAGE, and transferred to PVDF membrane. The Western blots shown are of two individual samples that detect the pool levels of total and reduced Trx. Western blot analyses show the level of reduced Trx and total Trx in young wild-type and dwarf fibroblasts. The upper band represents reduced Trx pool levels; the lower band represents total Trx pool levels. A similar pattern has been observed in three separate experiments. * p<0.05 between wild type and dwarf.