Figure 4.The pool levels of total thioredoxin and re-duced thioredoxin in fibroblasts derived from young wild type and dwarf mice. Cytoplasmic
extracts (100 μg) of young wild
type and dwarf fibroblasts were either untreated or treated with
4-acetamido-4'-maleimideyl-stilbene-2,2'-disulfonic acid (AMS) for 2 hours,
resolved by15% SDS-PAGE, and transferred to PVDF membrane. The Western
blots shown are of two individual samples that detect the pool levels of
total and reduced Trx. Western blot analyses show the level of reduced Trx
and total Trx in young wild-type and dwarf fibroblasts. The upper band
represents reduced Trx pool levels; the lower band represents total Trx
pool levels. A similar pattern has been observed in three separate
experiments. * p<0.05
between wild type and dwarf.
