Figure 3.ROS generating inhibitors of electron transport chain complexes, CI, CII and CIII, and H 2O2 affect the stability and levels of reduced thioredoxin-ASK1 complex [(SH)2Trx-ASK1]. The effects
of: (A) CI inhibitor, rotenone, (5 μM and 20 μM
for 60 minutes). (C) CIII inhibitor, antimycin A, (50 μM for 120 minutes), and (D)
H2O2 treatment (1 mM for 30 minutes) on the levels of
the (SH)2Trx-ASK1 complex in young (4-6 mos) wild-type and dwarf
fibroblasts. Western blot analysis below each of the bar graphs (A-D)
show the release of Trx bound to ASK1 as indicated by the amount of Trx
co-immunoprecipitated with anti-ASK1 antibody after treatment with ROT,
3NPA, AA, and H2O2.
