Research Paper Volume 1, Issue 9 pp 784—802

Figure 3. ROS generating inhibitors of electron transport chain complexes, CI, CII and CIII, and H ₂O₂ affect the stability and levels of reduced thioredoxin-ASK1 complex [(SH)₂Trx-ASK1]. The effects of: (A) CI inhibitor, rotenone, (5 μM and 20 μM for 60 minutes). (C) CIII inhibitor, antimycin A, (50 μM for 120 minutes), and (D) H₂O₂ treatment (1 mM for 30 minutes) on the levels of the (SH)₂Trx-ASK1 complex in young (4-6 mos) wild-type and dwarf fibroblasts. Western blot analysis below each of the bar graphs (A-D) show the release of Trx bound to ASK1 as indicated by the amount of Trx co-immunoprecipitated with anti-ASK1 antibody after treatment with ROT, 3NPA, AA, and H₂O₂.