Research Paper Volume 1, Issue 9 pp 784—802

Dermal fibroblasts from long-lived Ames dwarf mice maintain their in vivo resistance to mitochondrial generated reactive oxygen species (ROS)

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Figure 2. Growth curves of young (3-4 mos), middle aged and old (20-24 mos) wild-type and Ames dwarf fibroblasts. (A) Fibroblasts of young (3-4 mos) wild-type and Ames dwarf mice (3x104); (B) middle aged (10-12 mos) wild-type and Ames dwarf mice (3x104) and (C) old (20-24 mos) wild type and Ames dwarf mice (1x104) were plated in triplicate in a 12-well culture plate. The cells from all cultures were harvested and counted daily for 8 days. Each time-point represents the average number of cells in 3 triplicated wells of cultures established from 3 individual mice. N = 3; *p<0.05 between wild-type vs. dwarf mice.