Research Paper Volume 1, Issue 9 pp 771—783
Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle
Figure 3. Diet-sensitive expression of SIRT3 and AMPK in muscle tissue. (A) Mice were fed NIH-31 standard feed ad libitum or NIH-31/NIA-fortified diet (Harlan Teklad) with a daily food allotment of 60% of the control mice to establish caloric restriction (CR); twelve months after the onset of CR, tissues were harvested to examine SIRT3 expression. (B) Mice were deprived of food for 24 hours, and SIRT3 level in EDL muscle was determined by Western blot analysis. (C) SIRT3 protein expression is decreased in murine hind-leg muscle after 3 months of high-fat diet feeding; total hind-leg tissue protein was isolated and analyzed. (D) AMPK T-172 phosphorylation and AMPK total protein in the quadriceps of the caloric restricted mice were assayed; AMPK phosphorylation was determined as phospho-AMPK normalized by total AMPK. N=3, *P < 0.05.