Research Paper Volume 1, Issue 7 pp 652—663

Figure 3. Total cytochrome c assay. A quantitative western blot assay was developed to measure total cytochrome c in serum. The assay employed denaturing and reducing conditions to disrupt cytochrome c binding to carrier proteins in serum. The assay utilized serial dilutions of purified cytochrome-c resolved by SDS PAGE and western blotting (a) to generate standard curves (b) by digitally imaging and quantifying the chemiluminescent signal and serum from men (c) and women (d) were analyzed in parallel. Standards and serum samples were analyzed in duplicate.