Figure 3.Total cytochrome c assay. A quantitative
western blot assay was developed to measure total cytochrome
c in serum. The assay employed denaturing and reducing conditions to
disrupt cytochrome c binding to carrier
proteins in serum. The assay utilized serial dilutions of purified cytochrome-c resolved by SDS PAGE and western
blotting (a) to generate standard curves (b) by digitally
imaging and quantifying the chemiluminescent signal and serum from men (c)
and women (d) were analyzed in parallel. Standards and serum samples
were analyzed in duplicate.
