Figure 1.Up-regulation of gene expression with telomere shortening in human fibroblasts. mRNA levels of ISG15
and agrin were assayed by qantitative PCR
in human fibroblasts with different telomere lengths. Results from at least
three separate experiments are shown as means ± SEM. GAPDH was used as an
internal normalization control. All values were then normalized to the
level (=100%) of mRNA in young cells with long telomeres (PD18)
(see Table 1). Results show increase of ISG15 and agrin expression with telomere
shortening. Similar results for ISG15 were also obtained in IMR90 cells,
NHK and HME epithelial cells (Supplementary Figure 1).
