Figure 7.Apoptotic
markers in old mother cells (fraction V) of the mutant afo1Δ strain. (A)
phase contrast; (B) same cell as in A stained with Calcofluor White
M2R; (C) the same cell stained with DHE indicating a high level of
ROS; (D) an old mother cell stained with FITC-annexin V revealing
inversion of the plasma membrane; (E) the same cell as in (D)
shows absence of staining with propidium iodide revealing intact plasma
membrane; (F) TUNEL staining of old afo1Δ cells.