Research Paper Volume 1, Issue 6 pp 529—541

Figure 2. Increase in activity of male flies on low calorie food is mediated by dSir2. dSir^4.5/dSir^4.5 (A) and dSir^5.26/dSir^5.26 (B) homozygous dSir2 null male flies have significantly lower 24-hour spontaneous physical activity on 0.5X (brown) & 1.5X (orange) calorie diet at two different ages. The mobility was based on the mean mobility of 3 vials with 10 male flies each, and expressed as mean total activity per vial during 24 hours +/- SEM. Statistical significance was determined by using two-tailed Student's t-test with unequal variances.