Figure 3.Resveratrol suppresses autophagy independently of SIRT1.HEK293 cells
stably expressing GFP-LC3 were transfected with either a control siRNA (A) or an siRNA
directed against SIRT1 (B) for 72 hours. Subsequently, cells were
subjected to nutrient starvation with or without 50 μM resveratrol
(Res) treatment for 4 hours. 40X magnification fields have been cropped and
zoomed for ease of punctae visualization. (C) Quantification
of punctae/cell from (A) and (B) of 4 fields are represented as a
percentage of fed DMSO treated control siRNA cells. Error bars represent
s.d.m. (D) Representative
western blot showing typical knockdown of SIRT1 by siRNA transfection in
HEK293 GFP-LC3 cells.
