Figure 1.Resveratrol inhibits autophagy in mammalian cells. (A) NIH/3T3 cells stably expressing the GFP-LC3
fusion protein were subjected to nutrient withdrawal by
replacing growth media (Fed) with Earle's buffered saline
solution (Starved) and treated with either DMSO or 50 μM
resveratrol (Res) for 2 hours. Representative fields at
63X (oil immersion) magnification are shown. (B) Quantification
of punctae/cell in (A) of at least 4 fields per treatment
are represented as a percentage of the starved DMSO treated
cells. (C) HEK293 cells stably expressing the GFP-LC3 fusion
protein were subjected to starvation and either DMSO or 50 μM
Res for 6 hours. Representative fields at 40X magnification
are shown. (D) Quantification was performed on HEK293 cells
as in (B). Error bars represent s.e.m. * (p < 0.0022).
