Research Paper Volume 1, Issue 7 pp 637—651

Figure 6. Effects of p38b MAPK knockdown in ISCs and EBs on PVR-induced phenotypes. (A) Effect of D-p38b knockdown in ISCs/EBs on ectopic PVR-induced ISC proliferation. The PH3-positive cells in the midgut of the 3-day-old flies were counted. White bar, esg>+; black bar, esg>UAS-PVR; dark gray bar, esg>UAS-D-p38b^as;UAS-PVR; gray bar, esg>UAS-D-p38b^as. The number of PH3-positive cells detected per midgut of esg>+ flies was set as 1. P-values were calculated using Student's t-test. (B) Effect of D-p38b knockdown in ISCs and EBs on PVR-induced accumulation of large esg- and Delta-positive cells. The guts of 3-day-old flies were labeled with anti-Delta and anti-GFP. (a-a''), esg>+; (b-b''), esg>UAS-PVR; (c-c''), esg>UAS-D-p38b^as;UAS-PVR; (d-d''), esg>UAS-D-p38b^as. Overlay (DAPI, blue; anti-Delta, red; anti-GFP, green). Asterisk indicates EC-like large esg-positive cell. Arrow indicates Delta-positive cells. Scale bar, 5 μM. C. Effect of D-p38b knockdown in ISCs and EBs on PVR-induced Delta mRNA expression. Expression of Delta was measured by quantitative RT-PCR of dissected gut from 3-day-old flies. White bar, esg>+; black bar, esg>UAS-PVR; dark gray bar, esg>UAS-D-p38b^as;UAS-PVR; gray bar, esg>UAS-D-p38b^as. Expression was normalized to the expression of rp49. The level of Delta mRNA in the midgut of esg>+ flies was set as 1. P-values were determined using Student's t-test.