Figure 5.D-p38b MAPK plays a role in oxidative stress-induced aberrant Delta/ Notch signaling within the adult midgut. (A) Effect of
D-p38b knockdown in ISCs and EBs on oxidative
stress-induced accumulation of abnormal large esg- and
Su(H)GBE-positive cells within the adult midgut.
The midguts of 5-day-old esg>+;Su(H)GBE-lacZ (a-f) or
esg>UAS-D-p38bas;Su(H)-GEB-lacZ (g-l) flies exposed to
10 mM PQ in 1% sucrose (d-f and j-l) or 1% sucrose media
control (a-c and g-i) were labeled for 16 h with anti-GFP
(a, d, g, and j) and anti-?-gal (b, e, h, and k - red).
(c, f, i, and l) merged image. (DAPI, blue) Arrow indicates
EC-like large esg-GAL4. Asterisk indicates large Su(H)GBE-positive
cell. Scale bar, 5 µM. (B) Effect of D-p38b knockdown on
oxidative stress-induced increase of Delta expression.
The level of Delta mRNA was measured by quantitative RT-PCR
of dissected guts from 5-day-old esg>+, esg>UAS-D-p38bas flies
exposed to 10 mM PQ in 1% sucrose or 1% sucrose media control
for 16 h. Expression was normalized to the expression of rp49.
The level of Delta mRNA in midgut of 5-day-old flies was
set as 1. White bar, 1% sucrose media; black bar, 10 mM PQ
in 1% sucrose media. P-values were determined using Student′s t-test.
