Figure 3 — The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila

Research Paper|Volume 1, Issue 7|pp. 637–651

The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila

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Figure 3.Effect of D-p38b activity on colony size in heat-shock FLP-catalysed site-specific recombination. (A) Lineage-marking random dividing cells by Flp-out cassette. Genotype: a-a'', y,w,hs-FLP¹²²/+;actin>y+>gal4,UAS-GFP; b-b'', y,w,hs-FLP¹²²/UAS-D-p38b^as;actin>y+>gal4,UAS-GFP and y,w,hs-FLP¹²²/UAS-D-p38b⁺;actin>y+>gal4,UAS-GFP. All clones were marked with GFP (green) and Prospero (red). An asterisk represents non-enteroendocrine small cells. Arrow head represents a transient enterocyte colony. Arrow indicates large EC cell nuclei. Original magnification is 400x. (B) Histograms showing colony analysis in each genotype. Genotype: closed circular, y,w,hs-FLP¹²²/+;actin>y+>gal4,UAS-GFP; open triangle, y,w,hs-FLP¹²²/UAS-D-p38b^as;actin>y+>gal4,UAS-GFP, open circular, y,w,hs-FLP¹²²/UAS-D-p38b⁺;actin>y+>gal4,UAS-GFP.