Research Paper Volume 1, Issue 7 pp 637—651

Figure 2. Effect of D-p38b MAPK signaling on DNA synthesis of intestinal cells and ISC division. (A) Effects of D-p38b MAPK modulation on BrdU incorporation levels in the adult midgut. Twenty-five day-old flies expressing esg>+ (a and b), esg>UAS-D-p38b^as(c and b), p38b^EY11174 (e and f) or p38b^KG01337 (g and h) were fed on 0.2 mg/ml BrdU media for 4 days, and stained with anti-BrdU. Overlay (DAPI, blue; anti-BrdU, red). Asterisk indicates enlarged EC nuclei. Arrow indicates small ISC, EB or EE cell nuclei. Scale bar, 5 μM. Original magnification is 400x. (B) Effect of D-p38b MAPK activity on the number of PH3-positive cells within the adult gut. Number of PH3-positive cells detected per midgut of 5-, 30- and 60-day-old esg>+ or esg>UAS-D-p38b^as flies and 3- and 30-day-old control flies, p38b^EY11174 or p38b^KG01337. The number of PH3-positive cells detected per midgut of 5-day-old flies was set as 1. White bar, 5-day-old flies; gray bar, 30-day-old flies; black bar, 60-day-old flies. P-values were calculated using Student's t-test. (C) Effect of D-p38b MAPK activation on the number of PH3-positive cells. Number of PH3-positive cells in the midguts of 5-day-old flies carrying esg>+ or esg>UAS-D-p38b⁺ were analyzed. White bar, esg>+; gray bar, esg>UAS-D-p38b⁺. The number of PH3-positive cells detected per midgut of 5-day-old flies was set as 1. P-values were calculated using Student's t-test.