Research Paper Volume 1, Issue 7 pp 637—651
The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila
Figure 1. Increased expression of D-p38b in ISCs and EBs within aged gut. (A) Increased expression of D-p38b-lacZ reporter construct in the adult posterior midgut with age. The midguts of 5- and 30-day-old flies, including those with two copies of the D-p38b-lacZ, were examined by X-gal staining. Squared boxes are enlarged images. Arrow head indicates β-gal-positive cells. Original magnification is 400x. (B) Increased expression of D-p38b-lacZ reporter construct in the Delta-positive and neighboring cells in aged gut. The midguts of 30-day-old flies were examined with anti-β-gal and anti-Delta. Anti-β-gal, green; anti-Delta, red; DAPI, blue. Scale bar, 1 μM.