Research Paper Volume 1, Issue 4 pp 402—411
MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8
Figure 2. IRAK1 but not TRAF6 levels are reduced in HCA2 cells overexpressing miR146a and miR 146b. (A) Northern blot analysis of total RNA prepared from control (insertless virus-infected) HCA2 cells (cntrl), cells infected with a miR-146a-expressing virus (146a) and cells infected with a miR-146b-expressing virus (146b). 8 μg of total RNA was loaded in each lane. (B) Western blot analysis of total protein lysates prepared from proliferating cells (cntrl, PD32), or cells overexpressing miR-146a or miR-146b, and analyzed for IRAK1 (top panel) and TRAF6 (bottom panel). Actin protein levels served as a loading control.