Figure 5.Putative mechanism(s) by which DHEA inhibits TXNIP.SaOS2 (panel A)
were pre-incubated with Tamoxifen or CCP for one hour prior to addition of
DHEA. After an additional incubation for 48 hours, proteins were extracted
and probed by western blot for the expression of TXNIP and Sirt1. Panel B,
The cells were treated with different concentrations of glucose with or
without DHEA. After 48 hours in cultures, proteins were separated by
electrophoresis and probed for TXNIP. Staining with β-actin in
antibody was used as a loading control. Panel C, Effect of DHEA and
resveratrol on G6PD activity. Protein extract (100 μg) was incubated
with DHEA or resveratrol at the indicated concentrations, after 30 min at
37°C, the activity of G6PD was measured as described in the methods
section. Data represent average of four determinations +/- SE.
