Research Paper Volume 1, Issue 4 pp 412—424
Dual targeting of the antagonistic pathways mediated by Sirt1 and TXNIP as a putative approach to enhance the efficacy of anti-aging interventions
Figure 5. Putative mechanism(s) by which DHEA inhibits TXNIP. SaOS2 (panel A) were pre-incubated with Tamoxifen or CCP for one hour prior to addition of DHEA. After an additional incubation for 48 hours, proteins were extracted and probed by western blot for the expression of TXNIP and Sirt1. Panel B, The cells were treated with different concentrations of glucose with or without DHEA. After 48 hours in cultures, proteins were separated by electrophoresis and probed for TXNIP. Staining with β-actin in antibody was used as a loading control. Panel C, Effect of DHEA and resveratrol on G6PD activity. Protein extract (100 μg) was incubated with DHEA or resveratrol at the indicated concentrations, after 30 min at 37°C, the activity of G6PD was measured as described in the methods section. Data represent average of four determinations +/- SE.