Figure 2.HPV E7 maintains survivin protein and induces site-specific changes in histone H3 modifications.
(A) Histone H3 modifications at 24, 48 and 72 h post-transfection
with E6 or E7 siRNA. Equivalent exposures for each pair of immunoblots
shown. (B) Aurora B and survivin protein levels following E6 or E7
silencing. (C) Cell death after E6 or E7 siRNA treatment. (D)
Relative changes in S10P H3, survivin, p53 and p21 proteins in response to
E7 or E6 siRNA. (E) RT-PCR determinations of survivin and lamin A/C mRNA
levels in SiHa cells as indicated. (F) Immunoblots showing effect of
aurora B silencing on survivin protein levels at 48h post siRNA
transfection.
