Figure 3.WRN expression induces S/G2 arrest in sgs1 top3 cells. Logarithmically growing
cultures of sgs1 top3 strain transformed with YEp112SpGAL, YEp112SpGAL-WRN,
or YEp112SpGAL-SGS1, and the vector-transformed wild-type parental strain
were induced at 2% gal concentration for 6 h. Cultures were harvested,
processed for DAPI staining as described in "Materials and Methods" and
were observed using Axiovert 200 M microscope(Zeiss; 100x lens). Shown is the DAPI staining of the sgs1
top3 transformed with YEp112SpGAL (upper left) and with YEp112SpGAL-WRN
(upper right). Arrows show cells with undivided nuclei. Distribution of
the cells in G1 (single cells) and S/G2 (budded cells) is shown in lower
panel.
