Research Paper Volume 1, Issue 2 pp 219—233

Figure 2. WRN expression in sgs1 top3 restores the slow growth phenotype of top3. Panel A, sgs1 top3 strain transformed with YEp112SpGAL or YEp112SpGAL-WRN were streaked on an SC-Trp plate containing either 2% glu or 2% gal. As a control sgs1 top3 strain transformed with YEp112SpGAL-SGS1 was streaked on both the plates. Plates were incubated at 30°C for 2 days and then photographed. Panel B, Wild type parental strain W303-1A or sgs1 strain transformed with YEp112SpGAL or YEp112SpGAL-WRN were streaked on an SC-Trp plate either containing 2% glu or 2% gal. Plates were incubated at 30°C for 4 days and then photographed. Composition of the plates was as in Panel A and Panel B respectively. Panel C, Comparison of growth of sgs1 top3 strain transformed with YEp112SpGAL, YEp112SpGAL-WRN or YEp112SpGAL-SGS1. Logarithmically growing cultures of above mentioned strains were reinoculated at OD_0.05 in SC-Trp medium containing 2% gal and were incubated at 30°C. Growth of the cultures was followed by their absorbance at OD₆₀₀. The experiment was repeated twice in duplicate with similar results. Data represent the mean with standard deviations indicated by error bars.