Figure 2.WRN expression in sgs1 top3 restores the slow growth phenotype of top3.Panel A, sgs1 top3 strain transformed with YEp112SpGAL or YEp112SpGAL-WRN were
streaked on an SC-Trp plate containing either 2% glu or 2% gal. As a
control sgs1 top3 strain transformed with YEp112SpGAL-SGS1
was streaked on both the plates. Plates were incubated at 30°C for 2 days
and then photographed. Panel B, Wild type parental strain
W303-1A or sgs1 strain transformed with YEp112SpGAL or
YEp112SpGAL-WRN were streaked on an SC-Trp plate either containing 2% glu
or 2% gal. Plates were incubated at 30°C for 4 days and then
photographed. Composition of the plates was as in Panel A
and Panel B respectively. Panel C, Comparison
of growth of sgs1 top3 strain transformed with YEp112SpGAL,
YEp112SpGAL-WRN or YEp112SpGAL-SGS1. Logarithmically growing
cultures of above mentioned strains were reinoculated at OD0.05
in SC-Trp medium containing 2% gal and were incubated at 30°C. Growth of
the cultures was followed by their absorbance at OD600. The
experiment was repeated twice in duplicate with similar results. Data
represent the mean with standard deviations indicated by error bars.
