Figure 1.WRN fails to rescue the MMS and HU sensitivity of sgs1. Cultures of wild-type
parental strain (W303-1A) or sgs1 strain transformed with
YEp112SpGAL or YEp112SpGAL-WRN were grown to early log phase (OD
600
of ~0.6 to 0.8). Ten-fold serial dilutions of these cultures were spotted
onto SC-Trp plates containing 2% gal and either MMS or HU at the indicated
concentrations. Plates were incubated at 30°C for 3 days (control plates)
and 5 days (MMS or HU plates) and then photographed.
