Figure 5.Sch9p mediates TOR-dependent increases in mitochondrial function.Comparative analysisof
mitochondria-related parameters in wild-type (wt), tor1Δ, sch9Δ and sch9Δtor1Δ strains in the DBY2006 genetic background.
(A) Mitochondrial oxygen consumption. (B) Mitochondrial
translation as described in Figure 1. (C) Western blot of the
Cox1p, Cox4p, porin and actin OXPHOS components in the four strains using
100 μg of whole cell extract in each
lane. We use the ratio of porin to actin as one measure of mitochondrial
abundance per cell (which is virtually the same between the strains) and
the ratio of Cox subunits to porin to demonstrate their specific increase
per mitochondrial mass.
