Research Paper Volume 1, Issue 1 pp 122—130

Figure 4. The ATM dependent damage response is not compromised in tumor samples. (A) Immunofluorescence of ATM autophosphorylation after ionizing irradiation. Splenocytes were irradiated with 5 Gy and microtome sections were prepared as described in the "Materials and Methods" section. ATM activation was measured by immunofluorescence with an antibody specific for the phosphorylation event at serine 1981. The left panels represent cells from a control animal, the right panel from an animal expressing the GFP-mTRF2 fusion. The upper panels are before, the lower panels after irradiation. (B) Western analysis of spleen from a secondary recipient mouse, which expressed GFP-mTRF2 and died from a CD4/CD8+/+ T cell lymphoma. Protein samples were probed with antibodies against GFP and mTRF2. g-Tubulin was included as a loading control. As a positive control for GFP expression protein extract from HeLa 1.2.11 cells expressing GFP-mTRF2 was loaded.