Figure 1.SIRT6 interacts with the DNA-PK DSB repair factor. (A) Endogenous SIRT6 protein associates with
large molecular weight complexes. HeLa cell nuclear extract (NE) was
separated by gel-filtration and fractions subjected to Western analysis
with SIRT6 antibody. Fractions with molecular weight standards are
indicated (arrows). (B) Coomassie stain of proteins in Flag-SIRT6
or negative control IPs from 293T cells. The identities of proteins
detected by mass spectrometry are indicated. (C) SIRT6, but not
SIRT1, associates with DNA-PK subunits. Western analysis of SIRT6, SIRT1,
and negative control IPs with the indicated antibodies. WCE: whole cell
extract. (D) SIRT6 interaction with DNA-PKcs, but not Ku70 and
Ku80, is resistant to ethidium bromide (EtBr). Western analysis as in (C)
except EtBr was added as indicated to disrupt DNA-mediated interactions. (E)
Endogenous interaction between SIRT6 and DNA-PKcs. Western analysis of
SIRT6-bound proteins in co-IPs from 293T cells.
