Figure 1.Increased level of 4‑HNE-protein adducts in C. elegans with silenced gst‑10 gene.
RNAi silencing of gst‑10 was carried out as previously described
[21] in C. elegans strain Bristol-N2; worms fed bacteria
transformed with an insert-free L4440 feeding vector served as the control.
The level of 4‑HNE-protein adducts was determined in 5-day-old worms
by competitive ELISA [93]. The values shown are means ± S.D. of 4
biological replicates (independently grown batches of worms) per group.
