Research Paper Advance Articles

Figure 4. qPCR validation of RNA-seq data. (A) qPCR expression analysis of 12 genes upregulated in aged RPE/choroid. (B) Relative mRNA expression levels of genes downregulated in aging and not differentially regulated in RPE/choroid from young and aged mice. Actin-B was used for normalization, and statistical analysis was performed using the unpaired t-test (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ns: nonsignificant). Data were presented as mean ± SEM.